Senior Professor of histology, embryology and cytogenetic at the Faculty of Health Sciences of the University of Abomey-Calavi (Benin).
Doctor Anatole LALEYE is a senior Professor of histology, embryology and cytogenetic at the Faculty of Health Sciences of the University of Abomey-Calavi (Benin). He has degrees in medical medicine before continuing his specialization in histology and cytogenetic sciences respectively at Catholic University of Louvain (Brussels) Belgium Victor Segalen Bordeaux II University (France). Considered as one of the father of cytogentic in Benin, he is the Director of the Laboratory of histology, embryology, cytogenetic and medical genetics as well as the Chairman of the Institute of Biomedical Sciences of Benin. Professor LALEYE focalized his research work in the area of human medical genetic and is a major figure in the evolution of cytogenetic in West Africa. His research work has centered around chromosomic diseases, immunogenetics (Diabetis), Renal Polykystosis and development of molecular screening tools. Out of research activities, he interests in filiation tests and teaching in many universities of Africa.
Professor LALEYE has published more than 73 papers with impact factors and has managed more than 20 theses. Professor LALEYE has been honored by the Republic of Benin as “Chevalier de l’Ordre National du Bénin”
Written by Razack OSSENI, PharmD, PhD, Toxicology University of Abomey-Calavi (Benin)
Title of the talk: Immunogenetic aspects of type 1 diabetes in Benin: pilot study
Kaossarath A.Fagbemi1,Thierry C. Marc Medehouenou2, Simon Azonbakin1, Marius Adjagba1, Razack Osseni1, Jocelyne Ahoueya1, Arnaud Agbanlinsou1, Raphael Darboux1 and Anatole Laleye1.
1Department of Biomedical Sciences, Laboratory of Cytogenetics and Medical Genetics, Faculty of Health Sciences, University of Abomey-Calavi, Benin
2Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Abomey-Calavi, Benin
Type 1 diabetes is a pathology in spectacular epidemiological progression both on the world and in Benin. It is a public health problem because of the heavy weight of managing cost and complications. The pathogenesis is multi factorial and complex, involving genetic, immunological and environmental factors.
The aims of this work was to study the involvement of the polymorphism of some genes of the HLA system in the development of type 1 diabetes in Benin. A retrospective approach was used to collect epidemiological data from diabetic patient records. The next step of the study which was prospective one, matched case-control study focused in patients recruiting and registering of patients with T1D and controls. Anti-GAD65 and C-peptide were assayed using radio immuno assay techniques on blood samples in patients with type 1 diabetes. HLA alleles were carried out using a PCR-SSP in case-control.
A total of 12,000 cases were studied, including 103 of T1D patients. The frequency of the T1D recovered was 0.9%. An overall mortality rate of 34% was observed in the group of patients with type 1 diabetes with 54.3% for men and 45.7% for women. Type 1 diabetes was significantly associated with HLA-DQA1*05:01, DQB1*02:01 alleles, HLA-DR3 haplotype and HLA-DR3-DR4 genotype. Anti-GAD65 was present in 74.5% of patients with type 1 diabetes. Statical analyzes has revealed a link between the high titles of anti-GAD65 firstly and C-peptide, the porter age of HLA-DR3 and HLA-DQA1*05:01 secondly.
This pilot work allowed us to know the immune genetic profile of some type 1 diabetic patients in Benin